咨詢： （重慶） （貴陽） Anti-Clathrin heavy chain 抗體 (ab21679)產品信息： - Product name:Anti-Clathrin heavy chain antibody
- Description
Rabbit polyclonal to Clathrin heavy chain - Tested applicationsWB, ICC/IF, IHC-P, Flow Cyt, IHC-FoFr, IP
- Species reactivityReacts with: Mouse, Rat, Hamster, Human
Predicted to work with: ChickenDoes not react with African Green Monkey - Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1650 to the C-terminus of Human Clathrin heavy chain. (Peptide available as ab23440.) - Positive control
- This antibody gave a positive signal in HeLa, Jurkat, A431 & NIH3T3 cells, as well as human kidney tissues sections.
Properties - FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 - Concentration Batch dependent within range: 100 µg at 0.3 - 1 mg/ml
- PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
- Signal Transduction
- Protein Trafficking
- Vesicle Transport
- Coat Proteins
- Tags & Cell Markers
- Subcellular Markers
- Organelles
- Caveolae and Clathrin
Applications Our Abpromise guarantee covers the use of ab21679 in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. | Application | Notes |
|---|
| WB | WB: Use a concentration of 1 µg/ml. Detects a band of approximay 180 kDa (predicted molecular weight: 180 kDa).Can be blocked with Human Clathrin heavy chain peptide (ab23440). | | ICC/IF | ICC/IF: Use a concentration of 1 µg/ml. | | IHC-P | IHC-P: Use at an assay dependent concentration. | | Flow Cyt | Flow Cyt: Use at an assay dependent concentration. | | IHC-FoFr | IHC-FoFr: Use at an assay dependent dilution. PubMed: 18852301 | | IP | IP: Use at an assay dependent concentration. |
Target - FunctionClathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network.
- Sequence similaritiesBelongs to the clathrin heavy chain family.
- Cellular localizationCytoplasmic vesicle membrane. Membrane > coated pit. Melanosome. Cytoplasmic face of coated pits and vesicles. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
Database links - Entrez Gene: 1213 Human
- Entrez Gene: 67300 Mouse
- Entrez Gene: 54241 Rat
- Omim: 118955 Human
- SwissProt: P53675 Human
- SwissProt: Q00610 Human
- SwissProt: Q68FD5 Mouse
- SwissProt: P11442 Rat
Alternative names - CHC17 antibody
- Clathrin heavy chain 1 antibody
- Clathrin heavy chain 2 antibody
Anti-Clathrin heavy chain antibody images  Immunocytochemistry/ Immunofluorescence - Clathrin heavy chain antibody (ab21679) ICC/IF image of ab21679 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab21679, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).  Western blot - Clathrin heavy chain antibody (ab21679) All lanes : Anti-Clathrin heavy chain antibody (ab21679) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252)
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)
Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Cytoplasmic lysate (ab14873)
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680 at 1/15000 dilution
Predicted band size : 180 kDa
Observed band size : 180 kDa  Immunoprecipitation - Clathrin heavy chain antibody (ab21679)This image is courtesy of an anonymous Abreview ab21679 immunoprecipitating clathrin heavy chain from human HeLa S3 whole cell lysate (20ug). A protein A matrix was used with the antibody at a concentration of 4ug/mg of lysate. ab21679 was further used in the western blot stage at a dilution of 1/250. The image shows: Lane 1: Clathrin IP (ab21679) Lane 2: Control IP Lane 3: Input (10%)  Immunoprecipitation - Clathrin heavy chain antibody (ab21679)This image is courtesy of an anonymous Abreview ab21679 immunoprecipitating clathrin heavy chain from rat PC12 whole cell lysate (20ug). A protein A matrix was used with the antibody at a concentration of 4ug/mg of lysate. ab21679 was further used in the western blot stage at a dilution of 1/250. The image shows: Lane 1: Clathrin IP (ab21679) Lane 2: Control IP Lane 3: Input (10%)  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Clathrin heavy chain antibody (ab21679) Image courtesy of Human Protein Atlasab21679 staining Clathrin heavy chain in human kidney. Paraffin embedded human (male) kidney tissue was incubated with ab21679 (1/7000 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab21679 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.  Flow Cytometry - Clathrin heavy chain antibody (ab21679)This image is a courtesy of Anonymous Abreview ab21679 staining Clathrin heavy chain in mouse embryonic fibroblast cells by Flow Cytometery. Samples were prepared by homogenization and centrifugation in trypsin and they were washed in PBS. Cells were fixed in 70% 40C ethanol and cell population was gated on Non-junk by FSC/SSC. The primary antibody was diluted 1/2000 in 1% BSA/PBS and incubated with sample for 1 hour at 22°C. An Alexa Fuor 488 conjugated goat polyclonal to rabbit IgG (H&L) was used as secondary antibody at dilution at 1/500.  Immunocytochemistry/ Immunofluorescence - Clathrin heavy chain antibody (ab21679)This image is a courtesy of Anonymous Abreview ab21679 staining Clathrin heavy chain in mouse embryonic fibroblasts by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde and permeabilized with -200C ethanol. Samples were incubated with primary antibody at 1/500 dilution (in 0.1%Saponin/1% BSA/PBS) for 1 hour. An Alexa Fluor®546-conjugated goat polyclonal to rabbit IgG (H+L) was used as secondary antibody at 1/500 dilution. Green color in image show positive staining with Alexa Fluor®546 conjugated secondary.
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Centrosome Marker100ul3996gamma Tubulinhystemcell一抗 Abcam ab11321Rabbit polyclonal to gamma Tubulin - Centrosome Marker100ul3996gamma Tubulinhystemcell一抗 Abcam ab11322Mouse monoclonal [TUB-11] to epsilon Tubulin100ul3996epsilon Tubulinhystemcell一抗 Abcam ab11323Mouse monoclonal [6-11B-1] to Tubulin （acetyl K40）100ul3996Tubulinhystemcell一抗 Abcam ab11324Mouse monoclonal [B3] to Tubulin - Polyglutamylated100ul4274Tubulinhystemcell一抗 Abcam ab11325Mouse monoclonal [TUB-1A2] to Tubulin100ul3996Tubulinhystemcell一抗 Abcam ab11326Mouse monoclonal [100/2] to alpha Adaptin100ul3996alpha Adaptinhystemcell一抗 Abcam ab11327Mouse monoclonal [100/1] to AP1+2 beta1 00ul3996beta 1/2 Adaptinhystemcell一抗 Abcam ab11328Mouse monoclonal [100/3] to gamma Adaptin100ul3996gamma Adaptinhystemcell一抗 Abcam ab11329Mouse monoclonal [AP180-I] to AP180100ul3996AP18hystemcell一抗 Abcam ab1133Rabbit polyclonal to GABARAP100ul3996GABARAPhystemcell一抗 Abcam ab11330Mouse monoclonal [C6G9] to Carcino Embryonic Antigen CEA200ul3996CEAhystemcell一抗 Abcam ab11331Mouse monoclonal [TD.1] to Clathrin heavy chain100ul3996Clathrin heavy chainhystemcell一抗 Abcam ab11333Mouse monoclonal [1B10] to Fibroblast Surface Protein 200ul3996Fibroblast Surface Proteinhystemcell一抗 Abcam ab11334Mouse monoclonal [LN-5] to Macrophage200ul3996Macrophagehystemcell一抗 Abcam ab11335Mouse monoclonal [45M1] to Gastric Mucin 200ul3996Mucin Gastrichystemcell一抗 Abcam ab11336Rabbit polyclonal to Parvin alpha50ul3996Parvin alphahystemcell一抗 Abcam ab11337Rabbit polyclonal to l Afadin100ul3996l Afadinhystemcell一抗 Abcam ab11338Rabbit polyclonal to l+S Afadin 100ul3996l/S Afadinhystemcell一抗 Abcam ab11339Mouse monoclonal [FA-5] to N Cadherin100ul3996N Cadherinhystemcell一抗 Abcam ab1134Rabbit polyclonal to GABA B Receptor 2100ul3996GABA B Receptor 2hystemcell一抗 Abcam ab11340Mouse monoclonal [GC-4] to N Cadherin100ul3996N Cadherinhystemcell一抗 Abcam ab11342Mouse monoclonal [CH-19] to pan Cadherin - Plasma Membrane Marker （FITC）100ul3996Cadherin panhystemcell一抗 Abcam ab11343Rabbit polyclonal to CASK100ul3996CASKhystemcell一抗 Abcam ab11346Rabbit polyclonal to alpha 1 Catenin100ul3885alpha 1 Cateninhystemcell一抗 Abcam ab11347Rabbit polyclonal to alpha 2 Catenin100ul3996alpha 2 Cateninhystemcell一抗 Abcam ab11348Mouse monoclonal [15B8] to beta Catenin （Cy3 ®）100ul3996beta Cateninhystemcell一抗 Abcam ab11349Mouse monoclonal [BC-76] to beta Catenin （phospho S33）50ul3996beta Cateninhystemcell一抗 Abcam ab1135Rabbit polyclonal to GABA B Receptor 1100ul3996GABA B Receptor 1hystemcell一抗 Abcam ab11350Mouse monoclonal [BC-22] to beta Catenin （phospho S33+S37）50ul3996beta Cateninhystemcell一抗 Abcam ab11351Rabbit polyclonal to delta 2 Catenin100ul3996delta 2 Cateninhystemcell一抗 Abcam ab11352Rabbit polyclonal to delta 2 Catenin100ul3996delta 2 Cateninhystemcell一抗 Abcam ab11354Mouse monoclonal [4C3] to HMGB150ug3996HMGB1hystemcell一抗 Abcam ab11355Mouse monoclonal [ICRF44] to CD11b50tests3996CD11bhystemcell一抗 Abcam ab11356Mouse monoclonal [ICRF44] to CD11b （FITC）50tests3996CD11bhystemcell一抗 Abcam ab11358Mouse monoclonal [A3D8] to CD4450tests3996CD44hystemcell一抗 Abcam ab11359Mouse monoclonal [8.4A6] to ICAM1100ul3996CD54,ICAM1hystemcell一抗 Abcam ab1136Rabbit polyclonal to Artemin100ul3996Arteminhystemcell一抗 Abcam ab11364Mouse monoclonal [FMC46] to CD62L100ul3996CD62Lhystemcell一抗 Abcam ab11365Mouse monoclonal [FMC-46] to CD62L （FITC）50tests3996CD62Lhystemcell一抗 Abcam ab11366Mouse monoclonal [CXN-32] to Connexin 32/GJB150ul3996Connexin 32,GJB1hystemcell一抗 Abcam ab11367Rabbit polyclonal to Connexin 32/GJB1100ul3996Connexin 32,GJB1hystemcell一抗 Abcam ab11368Rabbit polyclonal to Connexin 32/GJB1100ul3996Connexin 32,GJB1hystemcell一抗 Abcam ab11369Mouse monoclonal [CXN-6] to Connexin 43/GJA150ul3996Connexin 43,GJA1hystemcell一抗 Abcam ab11370Rabbit polyclonal to Connexin 43/GJA1100ul4418Connexin 43,GJA1hystemcell一抗 Abcam ab11371Mouse monoclonal [ZK-31] to Pan Desmocollin100ul3996Desmosomal Proteinhystemcell一抗 Abcam ab11372Mouse monoclonal [P1H12] to CD14650ul3996CD146hystemcell一抗 Abcam ab11375Rat monoclonal [YTH 8.18] to Macrophages+Neutrophils250ul3996Macrophage/Neutrophilshystemcell一抗 Abcam ab11379Mouse monoclonal [8E9] to Glucocorticoid Receptor100ug3996Glucocorticoid Receptorhystemcell一抗 Abcam ab1138Rabbit polyclonal to GRC5/PHF2100ul3996GRC5/PHF2hystemcell一抗 Abcam ab11380Mouse monoclonal [MAB 263] to Growth hormone receptor100ug3996Growth hormone receptorhystemcell一抗 Abcam ab11382Mouse monoclonal [INN-hCG-106] to hCG beta core fragment250ug3996hCG beta core fragmenthystemcell一抗 Abcam ab11386Mouse monoclonal [INN-hFSH-158] to Glycoprotein hormones （alpha 5）250ug3996Glycoprotein hormones （alpha 5）hystemcell一抗 Abcam ab11387Mouse monoclonal [INN-hCG-72] to hCG alpha 6250ug3996hCG alpha 6hystemcell一抗 Abcam ab11388Mouse monoclonal [INN-hCG-2] to hCG beta 1 epitope250ug3996hCG beta 1hystemcell一抗 更多詳情，請： 重慶市華雅干細胞技術有限公司 |
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